BM1258 PATHOLOGY AND MICROBIOLOGY LAB

ANNA UNIVERSITY CHENNAI: CHENNAI – 600 025
B.E DEGREE PROGRAMME :B.E. BIOMEDICAL ENGINEERING
(Offered in Colleges affiliated to Anna University)
CURRICULUM 2004 SEMESTER IV

BM1258 PATHOLOGY AND MICROBIOLOGY LAB

1. Haemoglobin Estimation.
2. Peripheral Smear Study.
3. Urine Smear Study
4. Cross matching of Blood.
5. Tissue Biopsy – Benign and Malignant.
6. Simple Stain test
7. Gram Stain test.
8. AFB Stain test.

List of equipment for a batch of 30

Sl.No	Description	Quantity required
1.	Haemoglobin Estimation Sahil’s Haemoglobinometer	1 set
2.	Peripheral Smear Study Peripheral smear – Routine Leishman’s Stain RBC – Size, Shape Inclusions in RBC WBC – Differential count Glass Slide, Binocular Microscope with light	1 set
3.	Urine Smear Study Urine Smear, Glass Slide, Cover slips Binocular Microscope with light	1 set
4.	Cross matching of Blood Blood Grouping : cross – matching with respective centisera available in the market Slide, Antisera, Binocular Microscope with light	1 set
5.	Tissue Biospy – Benign and Malignant Tissue Biospy – Aspirating biopsy needle (under doctor’s supervision) Glass slide	1 set
6.	Simple Stain test SIMPLE STAIN, Glass Slide Reagents – Methylene blue, Crystal Violet, Cabal Iuschin	1 set
7.	Gram Stain test SIMPLE GRAM STAIN Reagents – Crystal Violet, Gram’s Iodine, 95% ethyl alcohol Safranin Urine bacterial count/ml exceeding 100,000(10s) denotes urinary tract infection. Normal : 0-100 ml Glass Slide	1 set
8.	AFB Strain test ACID FAST STAIN (ZIEHL – NEELSON METHOD) Reagent – Carbal Iushin, Acid Alcohol, Methylene blue, Glass slide	1 set

FEEL USEFUL PLEASE GIVE +1