March 22, 2010

BM1258 PATHOLOGY AND MICROBIOLOGY LAB


ANNA UNIVERSITY CHENNAI: CHENNAI – 600 025
B.E DEGREE PROGRAMME :B.E. BIOMEDICAL ENGINEERING
(Offered in Colleges affiliated to Anna University)
CURRICULUM 2004 SEMESTER IV

BM1258 PATHOLOGY AND MICROBIOLOGY LAB

  1. Haemoglobin Estimation.
  2. Peripheral Smear Study.
  3. Urine Smear Study
  4. Cross matching of Blood.
  5. Tissue Biopsy – Benign and Malignant.
  6. Simple Stain test
  7. Gram Stain test.
  8. AFB Stain test.
List of equipment for a batch of 30

Sl.No

Description

Quantity
required

1.

Haemoglobin Estimation
Sahil’s Haemoglobinometer

1 set

2.

Peripheral Smear Study
Peripheral smear – Routine Leishman’s Stain
RBC – Size, Shape Inclusions in RBC
WBC – Differential count
Glass Slide, Binocular Microscope with light

1 set

3.

Urine Smear Study
Urine Smear, Glass Slide, Cover slips Binocular Microscope with light

1 set

4.

Cross matching of Blood
Blood Grouping : cross – matching with respective centisera available in the market Slide, Antisera, Binocular Microscope with light

1 set

5.

Tissue Biospy – Benign and Malignant
Tissue Biospy – Aspirating biopsy needle (under doctor’s supervision) Glass slide

1 set

6.

Simple Stain test
SIMPLE STAIN, Glass Slide
Reagents – Methylene blue, Crystal Violet, Cabal Iuschin

1 set

7.

Gram Stain test
SIMPLE GRAM STAIN
Reagents – Crystal Violet, Gram’s Iodine, 95% ethyl alcohol Safranin
Urine bacterial count/ml exceeding 100,000(10s) denotes urinary tract infection.
Normal : 0-100 ml
Glass Slide

1 set

8.

AFB Strain test
ACID FAST STAIN (ZIEHL – NEELSON METHOD)
Reagent – Carbal Iushin, Acid Alcohol, Methylene blue, Glass slide

1 set


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